All research products on this site, including all peptides, are sold strictly for laboratory and research use only, not for human or animal consumption, diagnostic, or therapeutic use. Our tallow skincare products are topical cosmetics. You must be 21 or older to enter.
By entering you confirm you are 21+ and accept these products are for research use only. See our Terms and Disclaimer.
Synthetic thymosin beta-4, 43 amino acids, lyophilized. Sold in 5mg vials and 50mg kits for laboratory research.
Complete the order
Supplies and compounds researchers add to this order
Identity, purity and handling data for this compound, in one place. Tap an identifier to copy it.
A third-party certificate for TB-500 is not published here yet. If your work needs one, ask us before ordering and we will tell you what testing is available for the current lot.
Ask about a certificateFor research use only. Not for human consumption.
TB-500 is sold here as synthetic thymosin beta-4 (Tβ4), the full 43-amino-acid peptide (CAS 77591-33-4, molecular weight about 4,963). Within it, the LKKTETQ motif (residues 17 to 23) is the central actin-binding sequence, and it is the region most studied for G-actin binding and cell migration in laboratory models.
Thymosin beta-4 is the most abundant G-actin-sequestering protein in eukaryotic cells, with intracellular concentrations estimated at 200-500 micromolar. It was originally characterized in thymic tissue but is now known to be expressed widely in platelets, neutrophils, cardiac tissue, and most other cell types. Its core biological activity involves maintaining the pool of unpolymerized actin available for rapid cytoskeletal remodeling in response to cellular signals.
Research into TB-500 and the broader thymosin beta-4 system has expanded significantly since studies in the early 2000s demonstrated that Tb4 is secreted from platelets at wound sites and exerts extracellular effects on tissue repair processes. Investigators have since examined angiogenesis, anti-inflammatory signaling, and cardiomyocyte protection in both in vitro and animal model contexts using TB-500 as a pharmacological probe.
The areas where this compound appears in the published research literature.
In vitro wound healing research uses standardized scratch assays in primary keratinocyte, fibroblast, or endothelial cell monolayers, quantifying cell migration rates into the wound area with time-lapse microscopy. Actin polymerization state is assessed by differential ultracentrifugation (G-actin vs. F-actin fractions) and fluorescence-labeled phalloidin staining of cytoskeletal organization.
Cardiac research employs isolated neonatal rat ventricular cardiomyocytes (NRVCMs) exposed to hypoxia/reoxygenation to model ischemic injury, with TB-500 introduced at defined concentrations. Outcome measures include viability assays (LDH release, live/dead staining), ILK and Akt phosphorylation by Western blot, and mitochondrial function via JC-1 staining. In vivo studies use coronary artery ligation in rodents with serial echocardiography to track cardiac function.
Angiogenesis studies use HUVEC tube formation assays on growth factor-reduced matrigel, quantifying tube length, junction number, and mesh area by automated image analysis. VEGFR2 phosphorylation and downstream ERK/Akt signaling are characterized by immunoprecipitation and Western blot, establishing the molecular basis of TB-500's endothelial cell effects in these laboratory systems.
Lyophilized TB-500 should be stored at -20°C, protected from light and moisture. Under these conditions, the lyophilized peptide maintains structural integrity for extended periods. Vials should be sealed and equilibrated to room temperature under desiccant before handling to prevent water vapor absorption.
Reconstituted TB-500 solutions for laboratory use should be prepared in sterile bacteriostatic water or PBS and held at 4°C for use within 48 to 72 hours. Researchers should prepare aliquots to avoid repeated freeze-thaw cycles, which promote peptide aggregation. Adsorption to low-binding plasticware is recommended when working at low nanomolar concentrations.
TB-500 (Thymosin Beta-4) for sale from Optimum ReGen Peptides, a US research-peptide company with a storefront in Medford, Oregon. It is in stock, priced from $35. Every order ships 2-Day Priority Air, a $13 flat rate anywhere in the US, with tracking, or can be picked up at the store.
TB-500 is supplied as lyophilized powder, for laboratory and research use only, not for human consumption.
